棕榈酸和硬脂酸对HepG2细胞血红素加氧酶-1和核转录因子Nrf2表达的影响

doi:10.11843/j.issn.0366-6964.2016.03.025

Abstract

Abstract:

The purpose of the study was to explore the effects of Palmitic acid (PA) and Stearic acid (SA) on genes and protein expression of heme oxygenase 1 (HO-1) and Nuclear Transcription Factor Nrf2 in HepG2 Cells.The HepG2 cells were treated with PA or SA of 0.25，0.5，0.75，1.0 mmol•L^-1 for 24 h，the mRNA and protein expression levels of HO-1 and Nrf2 were detected by quantitative reverse transcription PCR and western blotting，separately.The experimental results were as follows，compared with the control group，the mRNA and protein expressions of HO-1 and Nrf2 were significantly increased with the increasing of PA concentration (P＜0.01)；While the mRNA and protein expressions of HO-1 and Nrf2 were significantly increased with SA of the concentrations of 0.25，0.5，0.75 mmol•L^-1 (P＜0.01)，the increased amount of the mRNA and protein expressions of HO-1 and Nrf2 were relatively decreased when the concentration of SA was 1.0 mmol•L^-1.In conclusion，a relatively higher concentration of PA and a relatively lower concentration of SA can elevate the mRNA and protein expressions of HO-1 and Nrf2 significantly within 1 mmol•L^-1 in HepG2 cells.

CLC Number:

S852.2

HAO Li-hong，ZHANG Xiu-ying，ZHANG Jin-ming，CUI Xiao-xu，SUN Xiao-qi，GUAN Shu-han. Effects of Palmitic Acid and Stearic Acid on Genes and Protein Expressions of Heme Oxygenase 1 and Nuclear Transcription Factor Nrf2 in HepG2 Cell[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 603-608.

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Effects of Palmitic Acid and Stearic Acid on Genes and Protein Expressions of Heme Oxygenase 1 and Nuclear Transcription Factor Nrf2 in HepG2 Cell

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